Part:BBa_J18912:Design

T7 promoter + RBS + START

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 20
  

Design Notes

• sequence is taken from the commonly used pET3a expression vector

• deleted XbaI site between prom. and RBS

• T7 promoter sequence (1-19) equals the one in BBa_I712074

• Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.

Source

pET3a expression vector